Evaluation of Silver Nanoparticles Caffeic folate (NSFC) Complex Compound as New Potential Therapeutic Agent against Cancer Xenograft in Mice: Thesis Abstract

Thesis Abstract In the last few decades properties of silver compounds have been interest as potential cancer treatments. The present work was designed to study effect new conjugated caffeic and folic acid with nanoparticle definite molecular size applied without gamma radiation exposure, an antitumor agent against experimentally induced Ehrlich tumor attempted identify their mechanisms action throughout determination anti-tumor activities using MTT cytotoxic assay two human carcinoma cell lines in vitro, Apoptosis analysis by flow cytometry through caspase-8, caspase-3 TNF determine vivo . Adult female albino mice were used divided into five groups, Animals scarified following parameters estimated, glutathione, glutathione peroxidase, superoxide dismutase blood caspase8, caspase 3 from tissue. specimens processed for histopathological examination. Nano-silver folate (NSFC) complex compound treatment resulted a growth inhibition Hep-G2 MCF-7 cells (IC50 7.70 µM 14.50 µM, respectively). Flow cytometric revealed that exposed has apoptotic at caspases 8, more than any them alone. That disturbance found be associated kinetic induction apoptosis showed modulation antioxidant system beside its high percentage necrotic studies. novel synthetic Caffeic may potentially hope development breast therapeutics, which should attract further scientific pharmaceutical interest.